New Mexico State University

 

EPWS 310 - Plant Pathology

Syllabus - Fall 2016

 

Instructors : Rebecca Creamer; Office Skeen Hall N248; Phone 646-3068; Office Hrs 10:30-11:30 MWF

  Danielle Lara (TA); Office Skeen Hall W237; Office Hrs 11:30-1:30 M

 

Class Time and Location

Lectures: MWF 9:30-10:20, Skeen Hall W139

Laboratory: W 1:30-3:20, Skeen Hall W138

Work on collections after class hours is possible (call for access)

 

Description of Course

This course introduces you to the basic concepts of the description, cause, and control of plant disease. Representative plant diseases will be selected for the illustration of symptom development, epidemiology, physiological responses, prevention and control practices, and types of pathogenic agents. Emphasis will be placed on the influence of the environment, host genetics and physiology, cell-cell interactions, cellular responses and pathogen biology on disease occurrence, development, and epidemiology.

 

Textbook: Agrios G.N. 2005 Plant Pathology 5th edition, Academic Press: New York

 

Course Objectives

By the end of the course you should be able to do the following:

 

1. Identify the causes of plant diseases and explain the altered physiology associated with diseased plants.

2. Classify pathogens into orders and describe the biology of the pathogens.

3. Describe pathogen life cycles as they related to disease cycles.

Determine optimal disease management strategies based on pathogen and host biology

Predict the effect of environmental parameters and time on disease epidemiology.

Isolate and identify some plant pathogens and demonstrate that they cause disease.

Find accurate plant pathology related information and literature.

Critically analyze a plant pathology related problem.

Integrate information and knowledge from different sources.

Effectively communicate interpretations and findings.

 

Course Evaluation

Evaluation is based on the components listed below.

 

Three Lecture Exams

300 pts

 

Comprehensive Final Exam

100 pts

 

Quizzes

  40 pts

 

Plant disease topics

  20 pts

 

Disease case studies

  20 pts

 

Laboratory Exam

100 pts

 

Collection

100 pts

 

Laboratory Reports

  31 pts

 

Laboratory Journal

    9 pts

 

TOTAL

720 pts

 

Be present at all exams!!!!!!!

 

 

Late assignments

Lab reports, plant disease topics, or collections that are turned in after the specified date will result in a reduction of 10% of the total point value for each day it is late.

 

Missed Quizzes and Exams

Quizzes that are missed for any reason can not be re-taken or made up.  The lowest quiz grade will be dropped at the end of the semester.  Tests missed with certified medical excuses or prior instructor approval will be dealt with individually.

 

Academic Conduct

Please note that the following are considered academic misconduct by NMSU:

 

1.    Cheating or knowingly assisting another student in committing an act of cheating or other forms of academic dishonesty.  [This includes giving other students your collection organisms.]

2.    Plagiarism, including submitting examinations, themes, reports [including lab reports], drawings, laboratory notes, undocumented quotations, computer-processed materials [internet documents], or other material as one’s own work when such work has been prepared by another person or copied from another person.

 

Students may receive a zero grade (F) for plagiarized work, or for the entire course

 

Lecture Schedule

 

 

DATE

LECTURE / TOPIC

CHAPTER (Agrios)

 

August 17 – Sept 9

Introduction; History; Nature of disesase; Host Defense; Genetics; Physiology Control

1-7, 9

 

Sept 12

EXAM 1

 

 

Sept 14 - Oct 3

Fungi I: Introduction; Diseases caused by Myxomycetes, Plasmodiophoromycetes, Oomycetes, Chytridiomycetes and Zygomycetes; Introduction to Ascomycetes

Chapter 11

 

Oct 5

EXAM 2

 

 

Oct 7 - Oct 21

Fungi II: Diseases caused by Ascomycete and Basidiomycete

Chapter 11

 

Oct 24 – Oct 28

Viruses

Chapter 14

 

Oct 31

EXAM 3

 

 

Nov 2 - Nov 7

Bacteria

Chapter 12

 

Nov 9 - Nov 16

Parasitic Higher Plants; Nematodes

Chapters 10, 13, 15, 16

 

Nov 16 – Nov 30

Epidemiology

Chapter 8

 

Dec 2

Molecular Plant Pathology

 

 

Dec 2

Summary / Evaluation

 

 

Dec 5, 8-10 am

FINAL COMPREHENSIVE EXAM

 

Please note that if you have a disability that interferes with your academic progress, you may contact Services for Students with Disabilities at 646-6840 (voice) or 646-1918 (TTY) for an appointment. Services for Students with Disabilities is located in Garcia Annex, Room 102. Current appropriate documentation will be required to receive services.

 

 

Laboratory Sessions

Attendance at all laboratory sessions is REQUIRED. Evaluation of the laboratory portion of the class will be based on a final laboratory exam, three 2-4 page laboratory reports and a collection of 5 plant pathogens.

 

Laboratory Exam

100 pts

Collection*

100 pts

Laboratory Reports**

  31 pts

Laboratory Journal

    9 pts

 

 

* Each collection shalll include pure cultures of at least one obligate plant pathogen specimen and four specimens of fungal or bacterial plant pathogens (isolated from diseased plants). Each specimin shall be identified to genus.

 

** Three lab reports will be collected during the semester. Reports should be prepared for any three experiments denoted with an asterisk (*) in the following syllabus. Lab reports are due ONE WEEK after the results are collected. You will receive a model report to guide you. Reports should be typed (spell check required).

 

Laboratory schedule for Plant Pathology 310, Fall-2016.

 

August 17

Microscope Technique

- How to use and calibrate a microscope

 

 

 

- Introduction to plant disease

 

 

 

- Differentiation between fungi and bacteria

 

 

 

- Culture transfer

 

 

 

 

 

August 24

Aseptic Technique

- Isolation from diseased plant materiald

 

 

 

- Koch's Postulate experiment

 

 

 

 

 

September 18

Fungi I

- Growth of fungi on different media

 

 

 

- A look at representative fungi

 

 

 

- Koch's Postulate experiment

 

 

 

 

 

September 7

Fungi II

- Fungal isolation -baiting

 

 

 

- Koch's Postulate experiment

 

 

 

- Measure fungal growth and sporulation

 

 

 

 

 

September 14

Fungi III

- Powdery mildew identification

 

 

 

 

 

September 21

 

 

 

 

Contol I

- Baiting – part 2

- Look at representative fungi

- Koch’s postulates experiments

 

- Seed treatment and control

 

 

 

- Even more representative fungi

 

 

 

 

 

September 28

Fungi III

- Inoculation of resistant and susceptible peppers

 

 

 

- Observe treated seeds

 

 

 

- Still more representative fungi to look at

 

 

 

 

 

October 5

Bacteria I

- Crown Gall experiment

 

 

 

- Gram staining of pathogenic bacteria

 

 

 

- Observe/record symptoms on pepper plants

 

 

 

- First lab report due!!

 

 

 

 

 

October 12

Bacteria II

- Chile leaf spot experiment

 

 

 

- Record crown gall symptoms

 

 

 

 

 

October 19

Viruses I

- ELISA detection of plant viruses

 

 

 

- Second lab report due!!

 

 

 

 

 

October 26

Viruses II

- Mechanical transmission of viruses

 

 

 

- Three Collection Specimens Due!!

 

 

 

 

 

November 2

Viruses III

- Polymerase Chain amplification of virus nucleic acid

 

 

 

 

 

November 9

Nematodes

- Dissection of root knot nematode galls

 

 

 

- Observation of different plant pathogenic nematodes

 

 

 

- Third lab report due!!

 

 

 

 

 

November 16

Lab Final Review

- COLLECTIONS DUE!!!

 

 

 

 

 

November 30

Lab Practical

 

 

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